Purification of Green Fluorescent Protein Introduction:
Animals[ edit ] An animal chimera is a single organism that is composed of two or more different populations of genetically distinct cells that originated from different zygotes involved in sexual reproduction.
If the different cells have emerged from the same zygote, the organism is called a mosaic. Chimeras are formed from at least four parent cells two fertilised eggs or early embryos fused together.
Each population of cells keeps its own character and the resulting organism is a mixture of tissues. Cases of human chimerism have been documented. In nonidentical twins, chimerism occurs by means of blood-vessel anastomoses. The likelihood of offspring being a chimera is increased if it is created via in vitro fertilisation.
Tetragametic chimerism[ edit ] Tetragametic chimerism is a form of congenital chimerism. This condition occurs through the fertilisation of two separate ova by two sperm, followed by aggregation of the two at the blastocyst or zygote stages. This results in the development of an organism with intermingled cell lines.
Put another way, the chimera is formed from the merging of two nonidentical twins a similar merging presumably occurs with identical twins, but as their genotypes are not significantly distinct, the resulting individual would not be considered a chimera. As such, they can be male, female, or have mixed intersex characteristics.
For example, the chimera may have a liver composed of cells with one set of chromosomes and have a kidney composed of cells with a second set of chromosomes.
This has occurred in humans, and at one time was thought to be extremely rare, though more recent evidence suggests that it is not the case. Recent research shows most marmosets are chimeras, sharing DNA with their fraternal twins. The difference in phenotypes may be subtle e.
If the blastocysts are of opposite sex, genitals of both sexes may be formed: Most chimeras composed of both male and female cells probably do not have an intersex condition, as might be expected if the two cell populations were evenly blended throughout the body.
Often, most or all of the cells of a single cell type will be composed of a single cell line, i.
Genitalia produce the hormones responsible for other sex characteristics. The most noticeable are some male tortoiseshells and calicos although most male tortoiseshells have an extra X chromosome responsible for the colouration or animals with ambiguous sex organs.
The Lydia Fairchild case, for example, was brought to court after DNA testing apparently showed that her children could not be hers. Banana Oil Lab Report Jesse Bradford 7/10/14 MTWR Section Introduction In the banana oil lab we began with isopentyl alcohol + acetic acid isopentyl acetate + Water.
We needed for this experiment a hot plate, clamps, pipette, 5mL vial, caps, hoses and a thermometer.
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BIOL Combined Lab ReportAlicia White Expression and Purification of recombinant Green Fluorescent Protein (rGFP) from E. Coli u. Report abuse. Transcript of GFP Lab. Flowchart Mariah Jaouni What we wanted to accomplish In this lab, our goal was to get the GFP by itself.
We did this by first using transformation. Once we transferred the gene inside the bacteria, we then separated the GFP. Next, we prepared our proteins to come out of the bacteria and get become ready for.
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